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Massachusetts Institute of Technology Technical Associate I, Love Lab in Cambridge, Massachusetts

Technical Associate I, Love Lab

  • Job Number: 20173

  • Functional Area: Research - Scientific

  • Department: Koch Inst - Integrative Cancer Research

  • School Area: VP Research

  • Employment Type: Full-Time

  • Employment Category: Exempt

  • Visa Sponsorship Available: No

  • Schedule:

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    Information on MIT’s COVID-19 vaccination requirement can be found at the bottom of this posting.

TECHNICAL ASSOCIATE I, STRAIN GENERATION AND CHARACTERIZATION, Koch Institute for Integrative Cancer Research ( -Love Lab ( (two openings), to enable and execute experiments focused on microbial strain development. Will apply working knowledge of current techniques in synthetic and molecular biology. Duties will include molecular cloning for plasmid construction, production of competent cells and subsequent electroporation for strain creation, strain characterization by RT-PCR and next generation sequencing, shake flask cultivation of yeast for strain assessment, protein quantification and characterization following cultivation (including SDS-PAGE, Western blot, ELISA) and implementing and maintaining standard operating procedures and data records. Will work with a knowledgeable and friendly multidisciplinary team of scientists who are part of a larger initiative developing an integrated platform to manufacture therapeutic recombinant proteins using a microbial host. This will offer an opportunity to make visible and recognized contributions to scientific research and cross-train in new technical areas.

Job Requirements

REQUIRED: B.S. in relevant scientific field (engineering, chemistry, biochemistry, microbiology); two years’ specialized lab experience (may include experience gained as an undergraduate); experience with primer design, molecular cloning, and synthetic biology tools; background in protein analytical techniques (e.g., SDS-PAGE, Western blot, dot blot, ELISA); excellent analytical, organizational, interpersonal, and verbal and written communication skills; meticulous attention to detail; commitment to producing accurate and reproducibly high-quality work; awareness of basic safety issues/procedures for maintaining a safe work environment; and willingness to work with and maintain technical instruments/equipment. Seek a collaborative and proactive individual who is able to multitask and plan, organize, and coordinate multiple work assignments; liaise with investigators; and document effective protocols. PREFERRED: M.S.; background in microbial cultivation and sterile technique; experience with automated liquid handling; and a publication record. Job #20173 reposted 5/13/22