CRISPR Therapeutics Research Associate II, Editing Technologies in Cambridge, Massachusetts
CRISPR Therapeutics is a leading gene editing company focused on developing transformative gene-based medicines for serious diseases using its proprietary CRISPR/Cas9 platform. CRISPR/Cas9 is a revolutionary gene editing technology that allows for precise, directed changes to genomic DNA. CRISPR Therapeutics has established a portfolio of therapeutic programs across a broad range of disease areas including hemoglobinopathies, oncology, regenerative medicine, and rare diseases. To accelerate and expand its efforts, CRISPR Therapeutics has established strategic collaborations with leading companies including Bayer, Vertex Pharmaceuticals and ViaCyte, Inc. CRISPR Therapeutics AG is headquartered in Zug, Switzerland, with its wholly-owned U.S. subsidiary, CRISPR Therapeutics, Inc., and R&D operations based in Cambridge, Massachusetts, and business offices in San Francisco, California and London, United Kingdom.
CRISPR Therapeutics is seeking a highly motivated Research Associate II to join the Cellular Biology team within our Editing Technologies group. The successful candidate will focus on cell line engineering and gene editing for the development of novel gene-editing technologies to advance our in vivo and ex vivo therapeutic programs.
Perform routine cell culture of adherent and suspension cell lines to support cell line engineering
Engineer mammalian cells by introducing plasmid DNA, mRNA, gRNA and Cas9 RNP by appropriate methods, including cationic lipid-based transfection and electroporation
Perform routine molecular biology techniques employing engineered cell lines to support development of next-generation sequencing (NGS) assays for sequence determination and assessment of on-and off-target DNA editing
Apply standard biochemical and immunological techniques, including protein extraction, protein gel electrophoresis, conventional and capillary western blotting, ELISA, and flow cytometry
Perform standard molecular biology techniques including DNA extraction, RNA extraction, and PCR
Work under the direction of a Research Scientist and across other platform technology and therapeutic groups to achieve desired goals
Maintain detailed records and tracking of engineered cell line production and inventory
Communicate key findings with internal and external partners by written reports and oral presentations
BS degree in Molecular Biology, Cell Biology, Biochemistry or a related discipline, and 3+ years of relevant research experience, or MS degree in a similar field and 1+ year of research experience obtained through industry co-op/internship and/or thesis-related research
Proficiency with mammalian cell culture techniques, including maintenance of adherent and suspension cell lines, transfection, cell counting, and standard cellular assays
Proficiency with standard molecular biology techniques (isolation of DNA and RNA, PCR, ddPCR, gel electrophoresis, oligonucleotide primer design)
Experience with routine biochemical and immunological methods (protein extraction, western blotting, flow cytometry, ELISA)
Working knowledge of CRISPR/Cas gene editing in mammalian cells
Demonstrated ability to work collaboratively with peers and effectively in a team-oriented environment
Superior attention to detail and organizational skills
Excellent oral and written communication skills
Experience with culturing primary human cells
Experience with engineering stable eukaryotic cell lines
Familiarity with DNA analysis software (e.g., Geneious, Snapgene, or similar)
Experience with lentivirus and/or AAV production, and lentiviral transduction
Experience with RNA synthesis by in vitro transcription
Problem Solving –Understands a situation or task by breaking it down into smaller pieces using a systematic approach and identifies patterns and connections between situations that are not obviously related.
Creativity/Innovation - Creates a boundary-less environment that fosters curiosity and taking risks. Demonstrates openness to new approaches, diversity of new thought, blank page creativity, and opportunistic thinking.
Perseverance – Pursues tasks with energy, drive, and initiative, even in the face of adversity.
Organization / Attention to Detail – Maintains a highly organized focus on research work, demonstrates highly organized thinking and documentation, and complete follow-through. Pursues all activities with diligence and completeness.
CRISPR Therapeutics, Inc. is committed to equal employment opportunity and non-discrimination for all employees and qualified applicants without regard to a person's race, color, gender, age, religion, national origin, ancestry, disability, veteran status, genetic information, sexual orientation or any characteristic protected under applicable law.
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