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Children's Hospital Boston Research Assistant I - Pulmonary in Boston, Massachusetts

57668BRTitle:Research Assistant I - PulmonaryDepartment:Medicine-PulmonaryAutoReqId:57668BRStatus:Full-TimeStandard Hours per Week:40 Job Category:ResearchJob Posting Description:The Wang Lab at the Division of Pulmonary Medicine at Boston Children’s Hospital is seeking a highly motivated Research Assistant to support our basic research portfolio. The lab uses induced pluripotent stem cell technology and gene-editing techniques of CRISPR-Cas9 to study pediatric lung diseases, focusing mainly on cystic fibrosis. Successful candidate will also be working on one or more of the following projects: 1) Modeling various phenotypes of cystic fibrosis with iPSC-derived lung platform 2) Characterizing the function of iPSC-derived pulmonary ionocytes, 3) assessing how CF airway respond to SARS-CoV-2. The last project (3) is a joint collaboration with the BLS3 and BLS4 laboratories at the National Emerging Infectious Disease Laboratories (NEIDL), and candidate will be delivering cell cultures (prior to infection with the virus) to the NEIDL laboratories for SARS-CoV-2 infection by NEIDL trained staff. Candidate will then process safely-treated, non-infectious samples for down-stream analysis, and should not have any direct contact with live SARS-CoV-2 virus. The candidate will also have the opportunity to interact with world-class collaborators at the Harvard campus as well as outside institutions including Boston University.

This Research Assistant I will:

  • According to prescribed protocols and assigned schedules, perform a variety of routine laboratory experiments and procedures, utilizing standard laboratory equipment, recording and checking results for completeness and accuracy

  • Assist in troubleshooting procedures

  • Perform the following techniques typically required for the laboratory/study:

  • Perform induced pluripotent stem cell (iPSC) culturing, including freeze, thaw, culturing, mycoplasma testing

  • Perform directed differentiation of iPSCs to various tissue lineages, mainly lung epithelium (proximal airway, distal lung) and immune cells

  • Perform PCR, RT-PCR, and other common molecular biology techniques setup

  • Perform flow cytometry and cell-sorting

  • Perform immunohistochemistry

  • Perform microscopy, including confocal

  • Maintain records of results and methodologies in a log of work performed

  • Maintain equipment, lab area and supplies in a neat professional manner

  • Sterilize and clean glassware, prepare media and other materials using appropriate procedures

  • Inventory supplies, alert supervisor to needed supplies, or mix standard reagents

  • Attend courses in safety procedures for handling, storage and disposal of biohazardous, flammable, corrosive and toxic materials

  • Carry out all laboratory activities in compliance with applicable regulations and protocols

  • Conduct literature searches and reviews, compiling information for journals and suggesting uses for new techniques to the P.I.

  • Attend courses, lectures and conferences to further own knowledge of research and laboratory techniques, consulting with other researchers and technicians to exchange information

To qualify, you must have:

  • BS in Biology, Molecular Biology, Biochemistry or related field

  • Wet bench experience is desirable

  • Analytical skills to resolve problems that require the use of basic scientific, mathematical, or technical principles

During a public health emergency, individuals in this role may be expected to take on additional duties to respond to organizational needs.

BCH offers competitive compensation and benefits.

BCHis an Equal Opportunity/Affirmative Action Employer. Qualified applicants will receive consideration for employment without regard to their race, color, religion, national origin, sex, sexual orientation, gender identity, protected veteran status or disabilityOffice/Site Location:BostonRegular, Temporary, Per Diem:Regular